Novel Agrobacterium Strains That Will Transiently Express But Not Integrate T-DNA
Optimize plant genetics with a new agrobacterium strain that achieves T-DNA transfer and gene expression without integrating into the host genome.
Researchers at Purdue University have developed a new agrobacterium strain for genetic transformation in plants. Agrobacterium is used to append a region of transfer DNA (T-DNA) of tumor-induced (Ti-)plasmid to a plant, and T-DNA is processed from virD1 and virD2 virulence proteins to achieve genetic transformation. Traditionally, this process often leads to undesired genetic alterations. Purdue researchers have optimized a process for synthesizing a new agrobacterium strain to transfer T-DNA without integrating it into the plant genome.
Advantages:
-Gene Expression Without Genome Integration
-T-DNA Transfer to Plants
Potential Applications:
-Plant Genetics
-Agrobiosciences
Technology Validation: New mutations of virD2 have been studied
Recent Publication:
Dr. Stanton Gelvin's Project Webpage
https://www.bio.purdue.edu/People/faculty/gelvin/gelvinweb/completeproposal.html
TRL: 2
Intellectual Property:
Provisional-Gov. Funding, 2020-10-09, United States | Utility-Gov. Funding, 2021-10-06, United States | CIP-Gov. Funding, 2024-04-10, United States