Multiplexed Inductive Nanoelectrospray Ionization Mass Spectrometry System
Automated high throughput nanoelectrospray ionization mass spectrometry (nESI-MS) sample pre-treatment drastically cuts screening time from one minute to 1.4 seconds, accelerating pharmaceutical bio-assay and chemical analysis.
Mass spectrometry has many applications including: pharmaceutical development, environmental sampling analysis, and investigative forensics. One growing application of mass spectrometry is high throughput screening is bio-assaying in pharmaceuticals. The lengthy sample pre-treatment in traditional mass spectrometry methods creates a challenging bottleneck for its users.
Researchers at Purdue University have developed an automated high throughput design for nanoelectrospray ionization mass spectrometry (nESI-MS) sample pre-treatment. The pre-treatment time has been reduced from 1 minute in traditional LC-MS to 1.4 seconds for each sample.. This design requires a minimum of 100 nL and can be loaded into a 384 well plate for multiplexed detection. The technology has been used to quantify peptide BACE1 at concentrations as low as 300 nM.
Advantages:
-Faster screening of mass spectrometry samples
-Small volume samples
Potential Applications:
-High throughput mass spectrometry
-Chemical structure analysis
-Bio-assay in pharmaceuticals
TRL: 4
Intellectual Property:
Provisional-Patent, 2019-05-31, United States | Utility Patent, 2020-05-28, United States | CON-Patent, 2021-09-01, United States
Keywords: Mass spectrometry, high throughput screening, bio-assaying, pharmaceuticals, nanoelectrospray ionization, nESI-MS, sample pre-treatment, faster screening, small volume samples, chemical structure analysis, Chemistry and Chemical Analysis, Mass Spectrometry, Pharmaceuticals, Proteins